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- 產(chǎn)品名稱： Anti PIK3CA(E545G) Mouse Monoclonal Antibody
- 產(chǎn)品貨號(hào)： CST26208
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購(gòu)： 4800
- 數(shù)量：
庫存： 5
- 規(guī)格： 100μg
- 產(chǎn)品信息
- 如何訂購(gòu)
Catalog Number
CST26208
Gene Symbol
PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
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Description
Anti PIK3CA(E545G) Mouse Monoclonal Antibody
Background
Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogen
A synthetic peptide from the internal region of PIK3CA which includes the mutation of E545G, human origin.
Applications
ELISA, WB, IHC
Recommended Dilutions
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Recognizes E545G mutant, but not wild-type PIK3CA of vertebrates.
Immunoprecipitation/Western Blot
Western blot analysis of recombinant PIK3CA(E545G) and wildtype proteins. Purified His-tagged PIK3CA(E545G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).
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Immunofluorescence:
Immunofluorescence of cells expressing PIK3CA proteins with Anti PIK3CA(E545G) antibody. HEK293T cells were transfected with pCDNA3-GFP PIK3CA(WT) plasmid (left column) or pCDNA3-GFP PIK3CA(E545G) plasmid (right column), then fixed and stained with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.
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