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- 產(chǎn)品名稱： Anti Active Ras Mouse Monoclonal Antibody
- 產(chǎn)品貨號(hào)：
- 貨期： 0
- 價(jià)格與訂購(gòu)： 4800
- 數(shù)量：
庫(kù)存： 5
- 規(guī)格： 100μg
- 產(chǎn)品信息
- 如何訂購(gòu)
Catalog Number
CST26909
Gene Symbol
RAS
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Description
Anti Active Ras Mouse Monoclonal Antibody
Background
Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Immunogen
Recombinant full length protein of active H-Ras
Applications
IP, IHC
Recommended Dilutions
1 μg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Isotype
IgG
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
nti active Ras antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.
Immunoprecipitation/Western Blot
IP/WB analysis of active Ras protein. Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti active Ras mouse monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti Ras mouse monoclonal antibody (Cat. # 26014).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.
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